Anti-D-dimer (human)
Mouse monoclonal antibody
Cat.No. ABS 015-28
Preparation: Protein-A purified
Content: Available in 200 µL and 1 mL volumes, 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC
D-dimer is a fibrin degradation product, a small protein fragment present in the blood after a blood clot is degraded by fibrinolysis.
Purified human D-dimer adsorbed onto aluminum hydroxide gel.
ABS 015-28 binds human D-dimer. Cross-reacts with directly coated D-monomer but not with directly coated fibrinogen.
When tested in a pair with itself, with ABS 015-22 and with a fibrinogen-specific polyclonal antibody, the epitope appears to relate to the neo-epitope formed by cleavage of D-dimer from fibrin.
ABS 015-028 binds free D-dimer in solution. The ELISA dilution guideline applies to D-dimer coated directly at 0.3 µg/mL.
ABS 015-028 works as capture antibody in sandwich ELISA with biotinylated ABS 015-21 or -28 as detection antibody.
Biotinylated ABS 015-028 works as detection antibody in sandwich ELISA with ABS 015-22 or ABS 015-28 as capture antibody.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1/8000 | |
| Immunoblotting | Not determined | ||
| Immunohistochemistry | Not determined |
The dilution guideline for ELISA is based on use as detection antibody for antigen coated at 0.3 µg/mL. Users should determine the optimal dilutions for their own purposes.
